Opinions on Gram-negative bacteria

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Gram-negative bacteria are a class of bacteria that do not retain the crystal violet stain used in the Gram staining method of bacterial differentiation, making positive identification possible. The thin peptidoglycan layer of their cell wall is sandwiched between an inner cytoplasmic cell membrane and a bacterial outer membrane. After staining with crystal violet, an alcohol wash is applied which decolorizes the bacteria showing that their peptidoglycan layer is too thin to retain the stain and enabling identification. A counterstain (safranin or fuchsine) is then added which recolorizes the bacteria red or pink.

Gram-positive bacteria have a thicker peptidoglycan layer in their cell wall outside the cell membrane, which retains the crystal violet stain during the alcohol wash, so long as it is timed correctly. The counter stain may also be absorbed by gram-positive bacteria but the darker crystal violet stain predominates.


In the image below, you can see a graph with the evolution of the times that people look for Gram-negative bacteria. And below it, you can see how many pieces of news have been created about Gram-negative bacteria in the last years.
Thanks to this graph, we can see the interest Gram-negative bacteria has and the evolution of its popularity.

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